只因不小心被一只受过放射性感染的蜘蛛咬伤，平凡瘦弱的高中生彼得·本杰明·帕克（尼尔·帕特里克·哈里斯 Neil Patrick Harris 饰）的人生便发生了翻天覆地的变化。从此以后，他穿上红蓝相间的紧身服，戴上面具，凭借发射出的蜘蛛丝穿梭在喧哗闹市的每一个角落。“能力越大，责任越大”，彼得以蜘蛛侠的身份，和破坏城市及世界和平的恶势力作斗争。全新的征程，更强大的敌人，女武士鹿田、The Talon、电王（Ethan Embry 配音）、蜥蜴人（罗布·赞比 Rob Zombie 配音）、银貂（Virginia Madsen 配音）等可怕对手相继登场。蜘蛛侠从不退却，勇敢地迎上前去…… 本片为采用3D技术制作的新版动画。
Plot Summary:An updated version of the classic animated adventure series. After being bitten by a radioactive spider, young Peter Parker finds that he now has spider-like super powers. Hoping to use his new-found abiilties for wealth and fame, he lets his ego blind him to the needs of others, and indirectly causes the death of his uncle Ben when he refuses to help a police officer catch a fleeing criminal. Humbled by his failure, he resolves to use his talents for fighting crime, and becomes the superhero Spider-Man. While he fights assorted super-villains, Peter also must balance his personal life, including his girlfriend Mary Jane, his job as a photographer at the Daily Bugle, and a an editor who has convinced himself that Spider-man is a criminal that has to be brought down.
